A DNA sequence encoding the SARS-CoV-2 (LF.7) Spike RBD (YP_009724390.1, with mutations I332V, G339H, R346T, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, K444R, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, del483/483, E484K, F486P, Q498R, N501Y, Y505H)(Arg319-Lys529) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant LF.7). The purified protein was biotinylated in vitro.